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The Immune Cell Landscape in Kidneys of Lupus Nephritis Patients
Internal Dataset- Authors
- Arnon AraziDeepak A. RaoCeline BerthierAnne Davidson39 more author(s)...
- Description
Lupus nephritis is a potentially fatal autoimmune disease for which the current treatment is ineffective and often toxic. To develop mechanistic hypotheses of disease, we analyzed kidney samples from patients with lupus nephritis and from healthy control subjects using single-cell RNA sequencing. Our analysis revealed 21 subsets of leukocytes active in disease, including multiple populations of myeloid...
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KidneyLupus NephritisRNA-SeqSequence Analysis, RNA
- Access Rights
- Free to all with registrationApplication Required
- Local Expert
- Anne DavidsonArnon AraziBetty Diamond
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Investigations into SCAMP5, a candidate lupus risk gene expressed in plasmacytoid dendritic cells
Internal Dataset- Authors
- Mustafa GhanemAndrew ShihHimanshu VashisthaLatanya Coke4 more author(s)...
- Description
Objective: We have investigated the molecular function of SCAMP5, a candidate risk gene for SLE exclusively expressed in plasmacytoid dendritic cells (pDCs) among peripheral leucocytes. Methods: We tested the independence of the association in SCAMP5 with SLE by performing conditional analyses. We profiled the expression pattern of SCAMP5 among circulating leucocytes at the transcript and protein...
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Dendritic CellsLupus Erythematosus, SystemicMembrane ProteinsRNA-Seq
- Access Rights
- Free to All
- Local Expert
- Andrew ShihPeter Gregersen
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Proteomic and single-cell transcriptomic dissection of human plasmacytoid dendritic cell response to influenza virus
Internal Dataset- Authors
- Mustafa GhanemAndrew ShihHouman KhaliliEmily Werth4 more author(s)...
- Description
Plasmacytoid dendritic cells (pDCs) represent a rare innate immune subset uniquely endowed with the capacity to produce substantial amounts of type-I interferons (IFN-I). This function of pDCs is critical for effective antiviral defenses and has been implicated in autoimmunity. While IFN-I and select cytokines have been recognized as pDC secreted products, a comprehensive agnostic profiling of the...
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Influenza A Virus, H1N1 SubtypeInterferon Type IProteomicsRNA-Seq
- Access Rights
- Free to All
- Local Expert
- Andrew ShihPeter Gregersen
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IRF5 genetic risk variants drive myeloid-specific IRF5 hyper-activation and pre-symptomatic SLE
Internal Dataset- Authors
- Dan LiBharati MattaSu SongVictoria Nelson5 more author(s)...
- Description
The IRF5-SLE risk haplotype is associated with SLE disease severity. We hypothesized that neutrophils from healthy risk donors would carry a pathogenic gene signature compared to non-risk donors. To compare basal neutrophil gene signature between risk and non-risk healthy individuals, fresh whole blood was collected from healthy donors, and granulocytes were extracted from the remaining pellet following...
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Genetic Predisposition to DiseaseGenetic VariationInterferon Regulatory FactorsLupus Erythematosus, Systemic
- Access Rights
- Free to All
- Local Expert
- Betsy BarnesPeter Gregersen
